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Galenza, (26794610 - PubMed)

This paper explains how researches manipulated the diets of Drosophila to see the impact of dietary glucose on adult flies. The impacts of dietary glucose supplementation on adult flies, had significant effects on longevity, locomotion, energy stores, microfloral composition, and immunity. They wanted to know if the microbiota was required for the benefits of glucose on longevity.  They found that when they eliminated the micro-biome, the lifespan of the flies that had diets with elevated glucose were not affected. This suggests that glucose levels influence host longevity independently of the micro-biome. 

My Newby Blog On Cancer Metastasis In Drosophila

I'm still getting the hang of reading thick scientific publications, but I think that the opportunities that Drosophila provides for studying and endless amount of genetic events is amazing. I read the paper "Modeling tumor invasion and metastasis in Drosophila" this week. Genetic mutations have been isolated that lead to the increase in a specific type of tyrosine kinase. Tyrosine kinase is important in regulating the cell cycle and elevated levels have been reported in many different human cancer subjects. An important barrier that cancer must pass in order to begin a secondary site is it must develop the ability to pass through epithelium .he epithelia of the Drosophila laval wing disc is perfect for experimentation. The manipulating of the Drosophila ortholog that controls the kinase levels allows researchers to cause cancerous growth in the larva. Researchers can then investigate how it invades surrounding tissues.

Scialo 2015, PMID:4630862

      The common theory on lifespan suggests that aging and age-related diseases is due to metabolic stress. After years of constantly working, your body eventually burns out. However, an alternative theory has begun to arise suggesting that perhaps aging is caused by developmental processes continuing in adulthood. The results of this paper are able to dissociate aging with metabolic rate and instead links it with nutrient pathways.

Maimon 2011, PMCID: PMC3197100

The method for selecting, dissecting, and staining ovaries from Drosophila Larvae is outlined. As stated in the article, these dissections are particularly helpful because of the relevant models they serve for interactions between germ line stem cells and somatic cells. Ovaries were dissected from larvae in the third larval instar because little is know regarding the formation of GSCs and somatic cells during development. A video article attached to the paper showed the delicate dissection of Drosophila larvae ovaries. This video led to a similar dissection video called “Dissection of Drosophila Ovaries” by Lin Chin Wong and Paul Schedl of Princeton University. These dissections were performed on adult Drosophila females. Interestingly, the ovaries were attained not simply by removal of the intestines and trachea followed by carefully teasing out the ovaries (a difficult and not always successful method). Rather, the person performing the dissections removed almost the entire rear abdo ...

Temple 2015

After establishing the maternal transmission of Wolbachia bacteria, the article detailed that Wolbachia can be parasitic or mutualistic depending on their host. Many of the known phenotypic impacts of Wolbachia on their hosts are classified as “reproductive parasitism” and include embryonic mortality in offspring, male embryo killing, asexual daughter development, and feminization. The main purpose of this study was to determine other phenotypic impacts of Wolbachia—especially behavioral characteristics. The study was performed on Wolbachia infected, Wolbachia-free, and naturally infected Wolbachia Armadillidium vulgare (pill-bugs). Tested was the A. vulgare’s learning performance as well as memory performance. The study showed a significant association between Wolbachia bacteria and decreased learning and decreased memory performance. It was unclear from reading the study whether or not the presence of other bacteria types played an impact on the behavioral results, especially because ...

Kalra 2014, PMID: 25223796

Overview of Kalra, 2014 (PMID: 25223796)  Trade-off of ovarian lipids and total body lipids for fecundity and starvation resistance in tropical populations of Drosophila melanogaster 

Wong, 2014 PMID: 24577449

Microbiota can have diverse effects on their hosts nutrition. Bacteria can consume ingested nutrients, change nutrient allocation patterns, and provide nutrients to their host. The comparison between microorganism-free (axenic) host and a host with a defined microbiome (gnotobiotic) has become popular in order to test the effect these microbes are having on their host. However, these tests do not take into account that it could simply be a lack of nutrients that is causing the effect. In this study, Wong, Dobson, and Douglas present what happens when you add nutrients back into the food. They use the fruit fly Drosophila melanogaster as their host organism.